Avl 9120, 9, 9140, 9180, 9181 electrolyte analyzers. Measuring cardiac activity cellular dynamics international. Atcc technology assessment of roche electronic impedance. A novel high throughput assay for anthelmintic drug. The xcelligence system was used according to the instructions of the supplier roche applied science and acea biosciences. Roche applied science in partnership with acea biosciences inc. The difference between rtc and the human is that rtc solves the dispatching problem on a much larger scale.
This chapter provides a summary of the document rtca do178b, software. The following equipment, consumables, and software are required in addition to the materials specified in the icell cardiomyocytes2 users guide. Rtcado178b, software considerations in airborne systems and equipment certification, december 1, 1992. A guide for endometrial cancer cell lines functional assays. Our search filters and notifiers help you get the used immunoassay analyzers you need. Environmental protection agency office of air and radiation 6604j epa 402r92004, july 1992 revised table of contents disclaimer acknowledgements significant changes in this revision radon and radon decay product measurement methods section 1. Use the software on more than one workstation at a time. Roche accuchek inform ii operators manual pdf download.
Description download rtca do160f free in pdf format. Founded in 1935 to be the voice of the aviation industry, rtca is chartered by the faa to operate federal advisory committees, and serves as the premier venue for developing consensus among diverse, competing interests, producing performance standards, policy and operational recommendations that are used by the government as the basis for regulations, as well as priorities for. Rtca pdf is a notforprofit corporation formed to advance the art and science of. Here, we will describe the xcelligence rtca cell adhesion protocol for hek293 cells.
Results to observe the effect of cell number on proliferation and determine the optimum cell number for the cytotoxicity experiment, 4000, 8000, and 16,000 cells per well were seeded in the eplate view. It consists of a realtime cell analysis rtca station, a computer with provided software, and singleuse eplates in which cells are grown figure 1. The rtca sp station is located inside a standard cell culture incubator and serves to transmit signals from an eplate 96 to the rtca analyzer. This manual is intended to be complementary to the operators manual where detailed instructions for. The rtca cardio instrument software rtca software offers a wide variety of provide a general guidance. The rtca dp analyzer can automatically select wells for. The xcelligence system rtca, developed by roche applied science, uses microelectronic biosensor technology to do dynamic, realtime, labelfree, and noninvasive analysis of cellular events, including cell number change, cell adhesion, cell viability, cell morphology, and cell motility solly et al. Roche xcelligence rtca sp system room n7, anderson stuart pc2 facility funded by clive and vera ramaciotti foundations xcelligence rtca sp system operator manual rtca 1. Marcos vivas tells us about cobas infinity, in combination with a roche total lab solution, making the difference in his laboratory at asociacion espanola in uruguay. Introduction to the data analysis of the roche xcelligence system with rtca package jitao david zhang april 27, 2020 1 introduction to the xcelligence system the xcelligence system, also known as rtces system, which contains a series of real time cell analyzer rtca, is a labelingfree cellbased assay system integrating micro. Keyword, title, document number, category, committee, or issue date. This manual is intended to be complementary to the operators manual where detailed instructions for operation, maintenance and troubleshooting are provided.
Contact your roche representative for further information. Xcelligence, eplate, and acea biosciences are registered. By developing the instruments and reagents you rely on each day, we strive to support your next breakthrough so you can focus on what you do. Introduction manual version software version revision date amendments 03 6. The realtime cell analyzer rtca software included with the xcelligence system provides an efficient tool to measure key growth parameters, including the calculation of celldoubling times, and permits the graphical representation of acquired data sets previously reported in xcelligence application note no. The first step consisted of a background reading followed by regular user defined reads at 15 sec intervals for adult and l 3 stages of all helminths tested now referred to as worm tests and 25. The xcelligence system consists of four main components. Lightcycler 96 system software as smart and intuitive as you want it to be whether you are a qpcr novice or a seasoned expert, the lightcycler 96 software can accommodate your needswithout wasting your time learning a new software package. Import, analyze and visualize data from roche r xcelligence rtca systems. Radio technical commission for aviation services also known as the radio technical commission on aeronautics, the rtca investigates technical issues relating to aviation telecommunications and maintains an active interest in gps technologies. Import, analyze and visualize data from rocher xcelligence rtca systems. The beating pattern stabilizes approximately 12 14 days postplating icell cardiomyocytes to the eplate. The joint committee of rtca special committee 167 and eurocae. Lightcycler 96 realtime pcr system roche life science.
Rtcado178 software considerations in airborne systems and equipment certification. Xcelligence, eplate, and acea biosciences are registered trademarks of. Additionally, the equipment and software installations, including application training, are conducted by our knowledgeable staff. Introduction to the data analysis of the roche xcelligence. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. By following do178c, organizations can implement aeronautical software. Indoor radon and radon decay product measurement device protocols u.
Using xcelligence rtca instrument to measure cell adhesion. About us we believe everything in the internet must be free. See the rtca cardio instrument operators guide for specific instructions. Our vast inventory of used immunoassay analyzers is sourced by many oems, including siemens, advia, abbott laboratories, ventana, and others. Any unauthorized modification of the instrument entails the invalidity of the guarantee. The cobas liat pcr system brings labquality testing to the point of care, delivering accurate results in 20 minutes or less enabling you to make the best treatment decisions possible when your patients need it most pcr accuracy receive realtime pcr at the point of care quick, definitive results no need for interpretation fast, easy operation perform three simple steps. The immunotherapy module is designed to measure cytolysis of target tumor cells by immune effector cells and other molecules. Tissue culture bosch bosch institute the university of. Rent, distribute, license or sublicense the software. Every effort has been made to ensure that all the information contained in the rtca software manual is correct at the time of printing. The xcelligence rtca sp and dp systems are available in the center for. The following items can be easily searched by using the online store search box. Copy, sell, or otherwise transfer the software or assign its rights under this agreement, in whole or in part, to another party, except as specifically set forth in this agreement. Rtcado178 software considerations in airborne systems.
European society for medical oncology esmo 2019 congress highlights from roche. Realtime xcelligence impedance analysis of the cytotoxicity. Roche molecular biochemicals lightcycler operators manual. The total number of documents purchased is limited to twenty documents per day. Do178b, software considerations in airborne systems and. Nov 16, 2010 the rtca controller software roche inc. As an alternative to commercial software shipped along the system, the bioconductor package rtca provides several unique transformation normalization strategies and various visualization tools.
Benefit from a userfriendly interface, online monitoring, and various analysis options like. Pdf a guide for endometrial cancer cell lines functional. Instruction manual rapide p12 2015 rapide p122016 rapide p122017 rapide p12 evo rapide p10 rapide p10w rapide p10w evo rapid. We celebrate the students, the researchers, the managers who work tirelessly in the pursuit of discovery. Cell and genomics research instruments and reagents 2012. Ninemonth 2019 sales presentation without appendix. Warranty information on warranty conditions are specified in the sales contract. Select from our inventory results below or call us at 212 772 6992 ext. Early drug development investor relations event 2019.
Page 1 accuchek inform ii blood glucose monitoring system operators manual page 2 revision history manual version revision date changes version 1. Lightcycler technology is the most innovative and rapid possibility for carrying out and simultaneously evaluating pcr experiments. A novel high throughput assay for anthelmintic drug screening. Click here to have us contact you with a quote or for further assistance. Rtca software pro is an integrated software package for running and analyzing real time cell analysis data from xcelligence rtca dp, sp, and mp instruments. Rtctools software tools for modeling realtime control reference manual dirk schwanenberg, bernhard becker version. Real time control tools user manual deltares open software. The package imports realtime cell electrical impedance data into r. Roche xcelligence rtca dp system room n7, anderson stuart pc2 facility funded by prostate cancer foundation of australia. Measurement device protocols indoor radon and radon. Acea biosciences has launched xcelligence rtca software pro, a powerful software package for running and analyzing immunotherapy.
The rtca control unit receives the data measured by the rtca analyzer and uses the rtca software 2. Lightcycler software and license agreement, grant of licence continued you may not. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. Code of federal regulations, aeronautics and space, parts 1 to 59, revised as of january 1, 1997. Rtc is a powerful software package that dispatches trains using the same elements as a human dispatcher.
Tissue culture bosch bosch institute the university. A guide for endometrial cancer cell lines functional. We stock and service roche equipment, replacement parts and related systems both current and discontinued products. The xcelligence system monitors cellular events in real time without the incorporation of labels by measuring electrical impedance across interdigitated microelectrodes integrated on the bottom of tissue culture eplates. The service manual for the 9120, 30, 40, 80, and 81 electrolyte analyzers contains the technical information needed to ensure easy fault identification. Acea biosciences launches xcelligence rtca software pro to. Firsttime users can easily start generating data for all common applications in gene. Cells should be delivered in suspension along with the protocol for each experiment. Make sure the correct thermal block cycler 96well for f. Expedited shipping and repair services are available for your urgent requirements. Roche lightcycler 96 biotecon diagnostics biotecon.
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